Journal: Antioxidants (Basel, Switzerland)

Article Title: Inhibition of Oxidative Stress and ALOX12 and NF-κB Pathways Contribute to the Protective Effect of Baicalein on Carbon Tetrachloride-Induced Acute Liver Injury.

doi: 10.3390/antiox10060976

Figure Lengend Snippet: Figure 2. Protein expression levels involved ferroptosis were detected in the liver tissues of mice challenged with CCl4. (A) Protein expression levels of ALOX12, HO-1, COX-2, p21 and Nrf2 at 6 and 24 h identified by Western blotting analysis of liver tissues of mice treated with CCl4; the quantitative analysis is showed in (B) (n = 4). 6 h vs. ctrl, * p < 0.05, ** p < 0.01; 24 h vs. ctrl, # p < 0.05, ## p < 0.01. Ctrl: control.

Article Snippet: The following primary antibodies were used to probe the membranes: primary rabbit antibodies against p21 (1:1000), COX2 (1:1000), Nrf2 (1:1000), and HO-1 (1:1000) (ProteinTech Group, Inc., Chicago, IL, USA), mouse monoclonal antibody against ALOX12 (1:1000) (Abcam, Cabridge, MA, USA) and β-actin (1:1000) (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Western Blot, Control

Journal: Antioxidants (Basel, Switzerland)

Article Title: Inhibition of Oxidative Stress and ALOX12 and NF-κB Pathways Contribute to the Protective Effect of Baicalein on Carbon Tetrachloride-Induced Acute Liver Injury.

doi: 10.3390/antiox10060976

Figure Lengend Snippet: Figure 6. Effects of baicalein supplementation on apoptotic pathway in the liver tissues of mice challenged with CCl4. (A) Representative TUNEL-stained sections showing apoptosis in the liver tissue of mice. (B) Quantitative analysis of TUNEL positive rates (n = 4). (C,D) Activities of (C) caspases-3 and -9 (D) (n = 6). (E) The relative expression of Bax, GADD45a and p21 mRNAs in the liver tissues (n = 5). ** p < 0.01, compared to the control group; # p < 0.05 or ## p < 0.01, compared to the CCl4 only group. Bar = 100 µm. Bai: baicalein.

Article Snippet: The following primary antibodies were used to probe the membranes: primary rabbit antibodies against p21 (1:1000), COX2 (1:1000), Nrf2 (1:1000), and HO-1 (1:1000) (ProteinTech Group, Inc., Chicago, IL, USA), mouse monoclonal antibody against ALOX12 (1:1000) (Abcam, Cabridge, MA, USA) and β-actin (1:1000) (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: TUNEL Assay, Staining, Expressing, Control

Journal: Regulatory peptides

Article Title: Increased circulating angiotensin-(1-7) protects white adipose tissue against development of a proinflammatory state stimulated by a high-fat diet.

doi: 10.1016/j.regpep.2012.06.009

Figure Lengend Snippet: Fig. 3. Adipose tissue inflammatory markers. Western blot for COX2-epididymal adipose tissue (A), RT-PCR expression of IL-1β‐epididymal adipose tissue (B), SD immunohisto- chemical reaction for IL-1β in epididymal adipose tissue (C), TGR immunohistochemical reaction for IL-1β in epididymal adipose tissue (D), RT-PCR expression of TNF-α‐ epididymal adipose tissue (E), SD immunohistochemical reaction for TNF-α in epididymal adipose tissue (F), TGR immunohistochemical reaction for TNF-α in epididymal adipose tissue (G). Data are presented as mean±SEM; * pb0.05.

Article Snippet: The blot was probed with COX2 enzyme antibody (Cell Signaling, USA; 1:1000), diluted in 1× blocking buffer, washed in 1× TBS with 1% of Tween 20, and then incubated with either IRDye 800 anti-goat secondary antibody (Rockland Immunochemicals®, Gilbertsville, Pa.) diluted to 1:10,000 in blocking buffer followed by further washes with PBS-Tween 20 and one wash in TBS.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunohistochemical staining